Article
Determination of Butyrate Synthesis Capacity in Gut Microbiota: Quantification of but Gene Abundance by qPCR in Fecal Samples.
Biomolecules - 2 Sept 2021
Daskova Nikola, Heczkova Marie, Modos Istvan, Videnska Petra, Splichalova Petra, Pelantova Helena, Kuzma Marek, Gojda Jan, Cahova Monika
Abstract excerpt
Butyrate is formed in the gut during bacterial fermentation of dietary fiber and is attributed numerous beneficial effects on the host metabolism. We aimed to develop a method for the assessment of functional capacity of gut microbiota butyrate synthesis based on the qPCR quantification of bacterial gene coding butyryl-CoA:acetate CoA-transferase, the key enzyme of butyrate synthesis. In silico, we identified...
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