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Article

CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation.

Stem cell research - 1 Aug 2021

Boehnke Janik, Atakhanov Salim, Toledo Marcelo A S, Schüler Herdit M, Sontag Stephanie, Chatain Nicolas, Koschmieder Steffen, Brümmendorf Tim H, Kramann Rafael, Zenke Martin

Abstract excerpt

The chemokine CXCL4/platelet factor 4 (PF4) gene, a key player in myelofibrosis, was knocked out by CRISPR/Cas9 in induced pluripotent stem cells (iPS cells) of a polycythemia vera (PV) patient with JAK2 V617F mutation. Two CXCL4KO iPS cell lines with and without JAK2 V617F mutation (UKAi002-B-1 and UKAi002-A-1, respectively) were generated. CXCL4KO iPS cells showed deletion of exon 1 and complete loss of CXCL4...

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Identifiers

PMID: 34391098
DOI: 10.1016/j.scr.2021.102490

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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