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The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols

1 Jan 2021

Abstract excerpt

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay is one of the methods used to evaluate the viability of sperm. In the assay, a tetrazolium component (MTT) is converted into MTT formazan by some specific enzymes in the viable cells. The amount of formazan product in theory is directly correlated with the percentage of viable sperms. It is quantified by measuring the...

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PMID: 34307082
DOI: 10.5455/ovj.2021.v11.i2.9

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