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Article

Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessment

26 Mar 2003

Abstract excerpt

BACKGROUND: The one-step eosin-nigrosin staining technique for assessment of sperm vitality was developed in the 1950s for various mammalian species. Although commonly used on human sperm in semen, a validation for this use has not previously been published. METHODS: The technique was evaluated on 1235 consecutive semen samples. RESULTS: The one-step eosin-nigrosin staining technique gave valid results when...

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PMID: 12660276
DOI: 10.1093/humrep/deg199

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