Article
A Modified Method to Isolate Circulating Tumor Cells and Identify by a Panel of Gene Mutations in Lung Cancer.
Technology in cancer research & treatment - 1 Jan 2000
Wang Helin, Wu Jieqing, Zhang Qi, Hao Jianqing, Wang Ying, Li Zhuoran, Niu Hongrui, Zhang Hongtao, Zhang Shucai
Abstract excerpt
The CellSearch system is the only FDA approved and successful used detection technology for circulating tumor cells(CTCs). However, the process for identification of CTCs by CellSearch appear to damage the cells, which may adversely affects subsequent molecular biology assays. We aimed to explore and establish a membrane-preserving method for immunofluorescence identification of CTCs that keeping the isolated...
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