Article
Single-nucleotide-level mapping of DNA regulatory elements that control fetal hemoglobin expression.
Nature genetics - 1 Jun 2021
Cheng Li, Li Yichao, Qi Qian, Xu Peng, Feng Ruopeng, Palmer Lance, Chen Jingjing, Wu Ruiqiong, Yee Tiffany, Zhang Jingjing, Yao Yu, Sharma Akshay, Hardison Ross C, Weiss Mitchell J, Cheng Yong
Abstract excerpt
Pinpointing functional noncoding DNA sequences and defining their contributions to health-related traits is a major challenge for modern genetics. We developed a high-throughput framework to map noncoding DNA functions with single-nucleotide resolution in four loci that control erythroid fetal hemoglobin (HbF) expression, a genetically determined trait that modifies sickle cell disease (SCD) phenotypes....
Topics
- Anemia, Sickle Cell
- Base Pairing
- Base Sequence
- Cell Line
- DNA
- Epigenesis, Genetic
- Fetal Hemoglobin
- Gene Editing
- Gene Expression Regulation
- Genome, Human