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Direct identification and molecular characterization of zoonotic hazards in raw milk by metagenomics using Brucella as a model pathogen.

Microbial genomics - 1 May 2021

Grützke Josephine, Gwida Mayada, Deneke Carlus, Brendebach Holger, Projahn Michaela, Schattschneider Alexander, Hofreuter Dirk, El-Ashker Maged, Malorny Burkhard, Al Dahouk Sascha

Abstract excerpt

Metagenomics is a valuable diagnostic tool for enhancing microbial food safety because (i) it enables the untargeted detection of pathogens, (ii) it is fast since primary isolation of micro-organisms is not required, and (iii) it has high discriminatory power allowing for a detailed molecular characterization of pathogens. For shotgun metagenomics, total nucleic acids (NAs) are isolated from complex samples such...

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PMID: 33945456
DOI: 10.1099/mgen.0.000552
PMCID: PMC8209726

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