Application of multiplex PCR-based nanopore sequencing for rapid detection and surveillance of antimicrobial-resistant Mycoplasma bovis in Japanese dairy cattle.

Abstract excerpt

Mycoplasma bovis causes mastitis and respiratory disease, leading to economic losses in the dairy industry. Conventional culture and antimicrobial susceptibility testing require approximately 2 weeks, promoting empirical antimicrobial use and emergence of resistant strains, highlighting the need for rapid detection methods. Resistance-associated mutations have been detected in rrl and rrs (for macrolides,...

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