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Examination of the usefulness of next-generation sequencing in mixed DNA samples.

Legal medicine (Tokyo, Japan) - 1 Jul 2021

Momota Fumi, Tsuji Akiko, Ishiko Atsushi, Ikeda Noriaki

Abstract excerpt

The identification of individuals from mixed DNA samples is an important application of DNA typing. Although the discriminatory power of DNA profiling has improved dramatically, a limiting factor is that individuals cannot be identified via short tandem repeat (STR) analysis. We used next-generation sequencing (NGS) to examine the mixed DNA samples. Our results showed that STR nucleotide sequences and single...

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PMID: 33930717
DOI: 10.1016/j.legalmed.2021.101874

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