Article
Efficient generation of osteoclasts from human induced pluripotent stem cells and functional investigations of lethal CLCN7-related osteopetrosis.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Aug 2021
Rössler Uta, Hennig Anna Floriane, Stelzer Nina, Bose Shroddha, Kopp Johannes, Søe Kent, Cyganek Lukas, Zifarelli Giovanni, Ali Salaheddine, von der Hagen Maja, Strässler Elisabeth Tamara, Hahn Gabriele, Pusch Michael, Stauber Tobias, Izsvák Zsuzsanna, Gossen Manfred, Stachelscheid Harald, Kornak Uwe
Abstract excerpt
Human induced pluripotent stem cells (hiPSCs) hold great potential for modeling human diseases and the development of innovative therapeutic approaches. Here, we report on a novel, simplified differentiation method for forming functional osteoclasts from hiPSCs. The three-step protocol starts with embryoid body formation, followed by hematopoietic specification, and finally osteoclast differentiation. We observed...
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