Article
Ratatosk: hybrid error correction of long reads enables accurate variant calling and assembly.
Genome biology - 8 Jan 2021
Holley Guillaume, Beyter Doruk, Ingimundardottir Helga, Møller Peter L, Kristmundsdottir Snædis, Eggertsson Hannes P, Halldorsson Bjarni V
Abstract excerpt
A major challenge to long read sequencing data is their high error rate of up to 15%. We present Ratatosk, a method to correct long reads with short read data. We demonstrate on 5 human genome trios that Ratatosk reduces the error rate of long reads 6-fold on average with a median error rate as low as 0.22 %. SNP calls in Ratatosk corrected reads are nearly 99 % accurate and indel calls accuracy is increased by...
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