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Article

New Strategies to Overcome Present CRISPR/Cas9 Limitations in Apple and Pear: Efficient Dechimerization and Base Editing.

International journal of molecular sciences - 30 Dec 2020

Malabarba Jaiana, Chevreau Elisabeth, Dousset Nicolas, Veillet Florian, Moizan Julie, Vergne Emilie

Abstract excerpt

Despite recent progress, the application of CRISPR/Cas9 in perennial plants still has many obstacles to overcome. Our previous results with CRISPR/Cas9 in apple and pear indicated the frequent production of phenotypic and genotypic chimeras, after editing of the phytoene desaturase (PDS) gene conferring albino phenotype. Therefore, our first objective was to determine if adding an adventitious regeneration step...

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Identifiers

PMID: 33396822
DOI: 10.3390/ijms22010319
PMCID: PMC7795782

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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