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Effective double-digest RAD sequencing and genotyping despite large genome size.

Molecular ecology resources - 1 May 2021

Gargiulo Roberta, Kull Tiiu, Fay Michael F

Abstract excerpt

Obtaining informative data is the ambition of any genomic project, but in nonmodel species with very large genomes, pursuing such a goal requires surmounting a series of analytical challenges. Double-digest RAD sequencing is routinely used in nonmodel organisms and offers some control over the volume of data obtained. However, the volume of data recovered is not always an indication of the reliability of data...

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PMID: 33351289
DOI: 10.1111/1755-0998.13314

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