Article
Application of a Sanger-Based External Quality Assurance Strategy for the Transition of HIV-1 Drug Resistance Assays to Next Generation Sequencing.
Viruses - 17 Dec 2020
Jennings Cheryl, Parkin Neil T, Zaccaro Daniel J, Capina Rupert, Sandstrom Paul, Ji Hezhao, Brambilla Donald J, Bremer James W
Abstract excerpt
The National Institute of Allergy and Infectious Diseases (NIAID) Virology Quality Assurance (VQA) established a robust proficiency testing program for Sanger sequencing (SS)-based HIV-1 drug resistance (HIVDR) testing in 2001. While many of the lessons learned during the development of such programs may also apply to next generation sequencing (NGS)-based HIVDR assays, challenges remain for the ongoing...
Topics
- Base Sequence
- Consensus Sequence
- Drug Resistance, Viral
- Genome, Viral
- Genotype
- HIV Infections
- HIV-1
- High-Throughput Nucleotide Sequencing
- Humans
- Sequence Analysis, DNA