Performance of the currently available DIVA real‐time PCR assays in classical and recombinant lumpy skin disease viruses

Abstract excerpt

The use of live homologous vaccines to protect against lumpy skin disease virus (LSDV) infection requires the use of molecular tools to differentiate between infected and vaccinated animals (DIVA). In this study, the commercial real-time PCR assays; ID Gene™ LSD DIVA Triplex kit and Bio-T kit® LSD – DIVA, as well as published assays targeting the GPCR gene (Journal of Virological Methods, 249, 48–57) and ORF008...

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