Article
A real-time PCR screening assay for the universal detection of lumpy skin disease virus DNA
1 Jul 2019
Abstract excerpt
OBJECTIVE: The resurgence of lumpy skin disease virus isolates of different genotypic natures abolishes the accuracy of assays that target either vaccine or field strain genome. The aim of the present study was to develop a universal real-time PCR assay using TaqMan chemistry to cover field, vaccine, and recombinant strains of lumpy skin disease virus isolates. RESULTS: The PCR assay was designed based on a...
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