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Generation of paternal haploids in wheat by genome editing of the centromeric histone CENH3.

Nature biotechnology - 1 Dec 2020

Lv Jian, Yu Kun, Wei Juan, Gui Huaping, Liu Chunxia, Liang Dawei, Wang Yanli, Zhou Hongju, Carlin Ryan, Rich Randy, Lu Tiancong, Que Qiudeng, Wang Wen Chung, Zhang Xingping, Kelliher Tim

Abstract excerpt

New breeding technologies accelerate germplasm improvement and reduce the cost of goods in seed production1-3. Many such technologies could use in vivo paternal haploid induction (HI), which occurs when double fertilization precedes maternal (egg cell) genome loss. Engineering of the essential CENTROMERIC HISTONE (CENH3) gene induces paternal HI in Arabidopsis4-6. Despite conservation of CENH3 function across...

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PMID: 33169035
DOI: 10.1038/s41587-020-0728-4

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