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Article

Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the CRB1 locus.

Stem cell research - 1 Dec 2020

Tang Xiangcheng, Chen Zhigang, Tan Xuhua, Luo Lixia, Liu Xialin, Gong Lili, Li David Wan-Cheng, Liu Yizhi

Abstract excerpt

Mutations in the CRB1 gene reportedly cause early-onset autosomal recessive retinitis pigmentosa (RP), which can result in severe loss of vision at an early age. To investigate the mechanism of CRB1-knockout (CRB1-/-) induced RP, we generated a subline of H9 human embryonic stem cells harboring frame shift mutations in a homozygous state in exon 2 of the CRB1 gene. This subline expressed pluripotent stem cell...

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Identifiers

PMID: 33128952
DOI: 10.1016/j.scr.2020.102057

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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