Article
A Novel White-to-Blue Colony Formation Assay to Select for Optimized sgRNAs.
Molecular biotechnology - 1 Jan 2021
Wei Chaogang, Chen Tong, Zhang Yueyue, Wang Yanfeng, Shi Dai, Jiang Zhen, Li Kai, Xiao Li, Shen Junkang
Abstract excerpt
CRISPR/Cas9-mediated genome editing technology consists of a single-guide RNA (sgRNA), and the Cas9 endonuclease has the potential to treat genetic diseases in most tissues and organisms. In this system, the Cas9 protein can be directed to target genomic DNA sequences as "molecular scissors" with the guidance of sgRNAs. However, the target-specific activities of different sgRNAs are highly variable; thus, it is...
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