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Development of a real‐time PCR assay for detection and quantification of Streptococcus iniae using the lactate permease gene

21 Sept 2020

Abstract excerpt

Abstract The aim of this study is the development and evaluation of a rapid and accurate quantitative PCR (qPCR)‐based protocol for detection of zoonotic pathogen Streptococcus iniae in bacterial cultures and tissues of diseased fish. For this purpose, the lactate permease‐encoding (lldY) gene was selected as a target for the design of S. iniae‐specific primers based on comparative genomic analysis using 45...

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PMID: 32959452
DOI: 10.1111/jfd.13267

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