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Article

Development and evaluation of a quantitative polymerase chain reaction for aquatic Streptococcus agalactiae based on the groEL gene

18 Dec 2019

Abstract excerpt

The aim of this study was to develop a TaqMan quantitative polymerase chain reaction (qPCR), based on the Streptococcus agalactiae groEL gene, to specifically quantify levels of bacteria within samples derived from aquatic sources, particularly aquaculture. Enumeration of bacteria by qPCR was compared with culture‐based methods. The qPCR was sensitive to 33 isolates of S. agalactiae, representing 11 clonal...

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PMID: 31851413
DOI: 10.1111/jam.14556

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