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A modified two-color flow cytometry assay to quantify in-vitro reinvasion and determine invasion phenotypes at low Plasmodium falciparum parasitemia.

Experimental parasitology - 1 Nov 2020

Ngoh Ines Atuh, Anong Damian Nota, Fru Jerome Cho, Bojang Fatoumata, Mbye Haddijatou, Amambua-Ngwa Alfred

Abstract excerpt

Invasion of human red blood cells (RBCs) by Plasmodium parasites is a crucial yet poorly characterised phenotype. Two-color flow cytometry (2cFCM) promises to be a very sensitive and high throughput method for phenotyping parasite invasion. However, current protocols require high (~1.0%) parasitemia for assay set-up and need to be adapted for low parasitemia samples, which are becoming increasingly common in low...

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PMID: 32858043
DOI: 10.1016/j.exppara.2020.107969

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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