Article
Determination of In-House Limit of Detection of Cell Number for PCR Based EGFR Mutations Detection in Small Biopsies of Non-Small Cell Lung Cancer.
Annals of clinical and laboratory science - 1 Jul 2020
Xie Jialing, Rui Weiwei, Chen Xiaoyan, Shen Xia, Zhang Lei, Gu Yijin, Dong Lei
Abstract excerpt
Mutations in the EGFR gene are biomarkers that guide the targeted therapy of Non-Small Cell Lung Cancer (NSCLC). When the mutation assay was performed on small Formalin-Fixed, Paraffin-Embedded (FFPE) biopsies in which only a limited number of tumor cells were obtained, determining Limit of detection of cell number (LODCN) was helpful for quality control. An NCL-H1975 cell block was used to determine the range of...
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