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Article

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling.

Journal of visualized experiments : JoVE - 1 Aug 2020

Mittal Deepika, Mishra Shruti, Prajapati Ramgopal, Vadassery Jyothilakshmi

Abstract excerpt

Forward genetic screens have been important tools in the unbiased identification of genetic components involved in several biological pathways. The basis of the screen is to generate a mutant population that can be screened with a phenotype of interest. EMS (ethyl methane sulfonate) is a commonly used alkylating agent for inducing random mutation in a classical forward genetic screen to identify multiple genes...

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PMID: 32804167
DOI: 10.3791/61259

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