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Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter system.

STAR protocols - 18 Jun 2021

Sun Shujing, Zhang Xiaoyan, Chen Kong, Zhu Xiaohong, Zhao Yang

Abstract excerpt

Environmental stimuli evoke transient increases of the cytosolic Ca2+ level. To identify upstream components of Ca2+ signaling, we have optimized two forward genetic screening systems based on Ca2+ reporter aequorin. AEQsig6 and AEQub plants were used for generating ethyl methanesulfonate (EMS)-mutagenized libraries. The AEQsig6 EMS-mutagenized library was preferably used to screen the mutants with reduced Ca2+...

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Identifiers

PMID: 34041505
DOI: 10.1016/j.xpro.2021.100558
PMCID: PMC8144734

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