Molecular malaria surveillance using a novel protocol for extraction and analysis of nucleic acids retained on used rapid diagnostic tests.

doi:10.1038/s41598-020-69268-5

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Molecular malaria surveillance using a novel protocol for extraction and analysis of nucleic acids retained on used rapid diagnostic tests.

Scientific reports - 23 Jul 2020

Guirou Etienne A, Schindler Tobias, Hosch Salome, Donfack Olivier Tresor, Yoboue Charlene Aya, Krähenbühl Silvan, Deal Anna, Cosi Glenda, Gondwe Linda, Mwangoka Grace, Masuki Heavenlight, Salim Nahya, Mpina Maxmillian, Said Jongo, Abdulla Salim, Hoffman Stephen L, Nlavo Bonifacio Manguire, Maas Carl, Falla Carlos Cortes, Phiri Wonder P, Garcia Guillermo A, Tanner Marcel, Daubenberger Claudia

Abstract excerpt

The use of malaria rapid diagnostic tests (RDTs) as a source for nucleic acids that can be analyzed via nucleic acid amplification techniques has several advantages, including minimal amounts of blood, sample collection, simplified storage and shipping conditions at room temperature. We have systematically developed and extensively evaluated a procedure to extract total nucleic acids from used malaria RDTs. The...

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PMID: 32703999
DOI: 10.1038/s41598-020-69268-5
PMCID: PMC7378824

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