Article
Pluripotency-associated genes reposition during early embryonic developmental stages in pigs.
Animal science journal = Nihon chikusan Gakkaiho - 1 Jan 2000
Nguyen Hiep Thi, Nghia Nguyen Trong, Lien Nghiem Thi Ha, Dang-Nguyen Thanh Quang, Men Nguyen Thi, Viet Linh Nguyen, Xuan Nguyen Bui, Noguchi Junko, Kaneko Hiroyuki, Kikuchi Kazuhiro
Abstract excerpt
We examined the allelic expression and positioning of two pluripotency-associated genes, OCT4 and SOX2, and two housekeeping genes, ACTB and TUBA, in 4- and 8-cell porcine embryos utilizing RNA and DNA fluorescence in situ hybridization (FISH) in single blastomeres. The proportion of blastomeres expressing SOX2 bi-allelically increased from 45% at the 4-cell stage to 60% at the 8-cell stage. Moreover, in 8-cell...
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