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Article

Importance of validating antibody panels: Anti-PD-L1 clone binds AF700 fluorophore.

Journal of immunological methods - 1 Aug 2020

Hughes Michael J, McGettrick Helen M, Sapey Elizabeth

Abstract excerpt

Researchers routinely use antibodies to assess the expression levels of proteins on the surface or intracellularly in a variety of different cell types. In this current study we highlight the importance of careful validation of antibodies for analysis of protein expression by flow cytometry and how failure to do so can significantly impact the interpretation of the data generated leading to false-positive...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 32464141
DOI: 10.1016/j.jim.2020.112795
PMCID: PMC7378575

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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