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Article

Antibody validation of immunohistochemistry for biomarker discovery: recommendations of a consortium of academic and pharmaceutical based histopathology researchers.

Methods (San Diego, Calif.) - 1 Nov 2014

Howat William J, Lewis Arthur, Jones Phillipa, Kampf Caroline, Pontén Fredrik, van der Loos Chris M, Gray Neil, Womack Chris, Warford Anthony

Abstract excerpt

As biomarker discovery takes centre-stage, the role of immunohistochemistry within that process is increasing. At the same time, the number of antibodies being produced for "research use" continues to rise and it is important that antibodies to be used as biomarkers are validated for specificity and sensitivity before use. This guideline seeks to provide a stepwise approach for the validation of an antibody for...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24525140
DOI: 10.1016/j.ymeth.2014.01.018
PMCID: PMC4240800

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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