Detecting HbE Gene Using DNA Extracted from Urine Sediments by Chelex-plus-Heating Technique.

Abstract excerpt

Detecting βE-allele or hemoglobin E (HbE) gene by PCR generally uses DNA prepared from blood leukocytes. However, blood drawing is invasive and prone to injury and infection. The so-called Chelex-plus-heating protocol for DNA extraction from urine sediments was performed in this study. In this protocol, urine sediments were incubated at 37°C with Chelex-100 resin, followed by heating in boiling water for 20 min,...

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