Article
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.
BioTechniques - 1 Apr 1991
Walsh P S, Metzger D A, Higuchi R
Abstract excerpt
Procedures utilizing Chelex 100 chelating resin have been developed for extracting DNA from forensic-type samples for use with the PCR. The procedures are simple, rapid, involve no organic solvents and do not require multiple tube transfers for most types of samples. The extraction of DNA from semen and very small bloodstains using Chelex 100 is as efficient or more efficient than using proteinase K and...
Topics
- Chelating Agents
- DNA
- Forensic Medicine
- Genotype
- HLA-DQ Antigens
- Humans
- Male
- Polymerase Chain Reaction
- Resins, Synthetic
- Semen