Article
Probing biophysical sequence constraints within the transmembrane domains of rhodopsin by deep mutational scanning.
Science advances - 1 Mar 2020
Penn Wesley D, McKee Andrew G, Kuntz Charles P, Woods Hope, Nash Veronica, Gruenhagen Timothy C, Roushar Francis J, Chandak Mahesh, Hemmerich Chris, Rusch Douglas B, Meiler Jens, Schlebach Jonathan P
Abstract excerpt
Membrane proteins must balance the sequence constraints associated with folding and function against the hydrophobicity required for solvation within the bilayer. We recently found the expression and maturation of rhodopsin are limited by the hydrophobicity of its seventh transmembrane domain (TM7), which contains polar residues that are essential for function. On the basis of these observations, we hypothesized...
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