Article
Extracellular expression of mutant CotA-laccase SF in Escherichia coli and its degradation of malachite green.
Ecotoxicology and environmental safety - 15 Apr 2020
Xu Kai-Zhong, Ma Hui, Wang Ya-Jing, Cai Yu-Jie, Liao Xiang-Ru, Guan Zheng-Bing
Abstract excerpt
In this study, mutant CotA-laccase SF was successfully expressed in Escherichia coli by co-expression with phospholipase C. The optimized extracellular expression of CotA-laccase SF was 1257.22 U/L. Extracellularly expressed CotA-laccase SF exhibits enzymatic properties similar to intracellular CotA-laccase SF. CotA-laccase SF could decolorize malachite green (MG) under neutral and alkaline conditions. The Km and...
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