Article
Variant phasing and haplotypic expression from long-read sequencing in maize.
Communications biology - 18 Feb 2020
Wang Bo, Tseng Elizabeth, Baybayan Primo, Eng Kevin, Regulski Michael, Jiao Yinping, Wang Liya, Olson Andrew, Chougule Kapeel, Buren Peter Van, Ware Doreen
Abstract excerpt
Haplotype phasing maize genetic variants is important for genome interpretation, population genetic analysis and functional analysis of allelic activity. We performed an isoform-level phasing study using two maize inbred lines and their reciprocal crosses, based on single-molecule, full-length cDNA sequencing. To phase and analyze transcripts between hybrids and parents, we developed IsoPhase. Using this tool, we...
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