Article
A real-time PCR assay based on a specific mutation of PstS1 gene for detection of M. bovis strains.
Biologicals : journal of the International Association of Biological Standardization - 1 Mar 2020
Ji Lingyun, Jiang Yi, Li Guilian, Zhao Xiuqin, Wan Kanglin
Abstract excerpt
The Mycobacterium tuberculosis complex (MTBC) is composed of several genetically related and pathogenic mycobacterial species, including M. tuberculosis, M. bovis and M.africanum et al. In our previous study, we found that M. bovis strains had a unique SNP located in position 1055 in the sequence of the pstS1 gene in which a T was substituted by a C. In this study, specific primers and MGB probes were designed...
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