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Article

Improved specificity for detection of Mycobacterium bovis in fresh tissues using IS6110 real-time PCR

1 Jan 2011

Abstract excerpt

BACKGROUND: Culture of M. bovis from diagnostic specimens is the gold standard for bovine tuberculosis diagnostics in the USA. Detection of M. bovis by PCR in tissue homogenates may provide a simple rapid method to complement bacterial culture. A significant impediment to PCR based assays on tissue homogenates is specificity since mycobacteria other than M. bovis may be associated with the tissues. RESULTS:...

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PMID: 21867516
DOI: 10.1186/1746-6148-7-50

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