Article
Rotary catalysis of bovine mitochondrial F1-ATPase studied by single-molecule experiments.
Proceedings of the National Academy of Sciences of the United States of America - 21 Jan 2020
Kobayashi Ryohei, Ueno Hiroshi, Li Chun-Biu, Noji Hiroyuki
Abstract excerpt
The reaction scheme of rotary catalysis and the torque generation mechanism of bovine mitochondrial F1 (bMF1) were studied in single-molecule experiments. Under ATP-saturated concentrations, high-speed imaging of a single 40-nm gold bead attached to the γ subunit of bMF1 showed 2 types of intervening pauses during the rotation that were discriminated by short dwell and long dwell. Using ATPγS as a slowly...
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