Article
Switching sides-Reengineered primary charge separation in the bacterial photosynthetic reaction center.
Proceedings of the National Academy of Sciences of the United States of America - 14 Jan 2020
Laible Philip D, Hanson Deborah K, Buhrmaster James C, Tira Gregory A, Faries Kaitlyn M, Holten Dewey, Kirmaier Christine
Abstract excerpt
We report 90% yield of electron transfer (ET) from the singlet excited state P* of the primary electron-donor P (a bacteriochlorophyll dimer) to the B-side bacteriopheophytin (HB) in the bacterial photosynthetic reaction center (RC). Starting from a platform Rhodobacter sphaeroides RC bearing several amino acid changes, an Arg in place of the native Leu at L185-positioned over one face of HB and only ∼4 Å from...
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