Article
High yield of B-branch electron transfer in a quadruple reaction center mutant of the photosynthetic bacterium Rhodobacter sphaeroides.
Biochemistry - 5 Mar 2002
de Boer Arjo L, Neerken Sieglinde, de Wijn Rik, Permentier Hjalmar P, Gast Peter, Vijgenboom Erik, Hoff Arnold J
Abstract excerpt
A new reaction center (RC) quadruple mutant, called LDHW, of Rhodobacter sphaeroides is described. This mutant was constructed to obtain a high yield of B-branch electron transfer and to study P(+)Q(B)(-) formation via the B-branch. The A-branch of the mutant RC contains two monomer bacteriochlorophylls, B(A) and beta, as a result of the H mutation L(M214)H. The latter bacteriochlorophyll replaces...
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