Article
High-intensity sequencing reveals the sources of plasma circulating cell-free DNA variants.
Nature medicine - 1 Dec 2019
Razavi Pedram, Li Bob T, Brown David N, Jung Byoungsok, Hubbell Earl, Shen Ronglai, Abida Wassim, Juluru Krishna, De Bruijn Ino, Hou Chenlu, Venn Oliver, Lim Raymond, Anand Aseem, Maddala Tara, Gnerre Sante, Vijaya Satya Ravi, Liu Qinwen, Shen Ling, Eattock Nicholas, Yue Jeanne, Blocker Alexander W, Lee Mark, Sehnert Amy, Xu Hui, Hall Megan P, Santiago-Zayas Angie, Novotny William F, Isbell James M, Rusch Valerie W, Plitas George, Heerdt Alexandra S, Ladanyi Marc, Hyman David M, Jones David R, Morrow Monica, Riely Gregory J, Scher Howard I, Rudin Charles M, Robson Mark E, Diaz Luis A, Solit David B, Aravanis Alexander M, Reis-Filho Jorge S
Abstract excerpt
Accurate identification of tumor-derived somatic variants in plasma circulating cell-free DNA (cfDNA) requires understanding of the various biological compartments contributing to the cfDNA pool. We sought to define the technical feasibility of a high-intensity sequencing assay of cfDNA and matched white blood cell DNA covering a large genomic region (508 genes; 2 megabases; >60,000× raw depth) in a prospective...
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