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Article

Effects of sampling strategy and DNA extraction on human skin microbiome investigations

21 Nov 2019

Abstract excerpt

The human skin is colonized by a wide array of microorganisms playing a role in skin disorders. Studying the skin microbiome provides unique obstacles such as low microbial biomass. The objective of this study was to establish methodology for skin microbiome analyses, focusing on sampling technique and DNA extraction. Skin swabs and scrapes were collected from 9 healthy adult subjects, and DNA extracted using 12...

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PMID: 31754146
DOI: 10.1038/s41598-019-53599-z

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