Article
[Establishment of a congenital chloride diarrhea-associated SLC26A3 c.392C>G (p.P131R) polymorphism-expressing cell model and a preliminary analysis of its mechanism of action].
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics - 1 Nov 2019
Zhang Ni-Ni, Guo Hong-Wei, Lin Yan, Zhang Wei, Zhang Wei, Wang Bao-Xi, Jiang Xun
Abstract excerpt
OBJECTIVE: To establish a congenital chloride diarrhea (CCD)-associated SLC26A3 c.392C>G (p.P131R) polymorphism-expressing cell model, and to investigate its biological function. METHODS: The sequence of the SLC26A3 gene in GenBank was used to design the upstream and downstream single-guide RNA (sgRNA) that could specifically recognize the 392 locus of the SLC26A3 gene, and the sgRNA was mixed with the...
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