Article
Validation of regulated protein phosphorylation events in yeast by quantitative mass spectrometry analysis of purified proteins.
Proteomics - 1 Oct 2012
Reiter Wolfgang, Anrather Dorothea, Dohnal Ilse, Pichler Peter, Veis Jiri, Grøtli Morten, Posas Francesc, Ammerer Gustav
Abstract excerpt
Global phosphoproteomic studies based on MS have generated qualitative and quantitative data describing protein phosphorylation events in various biological systems. Since high-throughput data for protein modifications are inherently incomplete, we developed a strategy to extend and validate such primary datasets. We selected interesting protein candidates from a global screen in yeast and employed a modified...
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