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Amplicon sequencing provides more accurate microbiome information in healthy children compared to culturing

5 Aug 2019

Abstract excerpt

Abstract Next-Generation Sequencing (NGS) of 16S rRNA gene is now one of the most widely used application to investigate the microbiota at any given body site in research. Since NGS is more sensitive than traditional culture methods (TCMs), many studies have argued for them to replace TCMs. However, are we really ready for this transition? Here we compare the diagnostic efficiency of the two methods using a large...

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PMID: 31396571
DOI: 10.1038/s42003-019-0540-1

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