Identification of O‐acetylhomoserine sulfhydrylase, a putative enzyme responsible for methionine biosynthesis in Clostridioides difficile: Gene cloning and biochemical characterizations

Abstract excerpt

Abstract O‐acetylhomoserine sulfhydrylase (OAHS) is a pyridoxal 5′‐phosphate‐dependent enzyme involved in microbial methionine biosynthesis. In this study, we report gene cloning, protein purification, and some biochemical characteristics of OAHS from Clostridioides difficile. The enzyme is a tetramer with molecular weight of 185 kDa. It possesses a high activity in the reaction of L‐homocysteine synthesis,...

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