Article
Bromphenol blue: nitrate reductase activity in Nicotiana plumbaginifolia: an immunochemical and genetic approach.
Biochimie - 1 Jan 2000
Meyer C, Cherel I, Moureaux T, Hoarau J, Gabard J, Rouze P
Abstract excerpt
NADH: nitrate reductase (EC 1.6.6.1) was purified from Nicotiana plumbaginifolia leaves. As recently observed with nitrate reductase from other sources, this enzyme is able to reduce nitrate using reduced bromphenol blue (rBPB) as the electron donor. In contrast to the physiological NADH-dependent activity, the rBPB-dependent activity is stable in vitro. The latter activity is non-competitively inhibited by NADH....
Topics
- Antibodies, Monoclonal
- Antibody Specificity
- Bromphenol Blue
- Enzyme-Linked Immunosorbent Assay
- Immunoglobulin G
- Kinetics
- Mutation
- Nitrate Reductases
- Phenols
- Plants, Toxic
- Nicotiana