Article
Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing
3 Jun 2019
Abstract excerpt
Detection and sequencing of chikungunya virus (CHIKV) genome was performed using a combination of a modified reverse transcription loop-mediated isothermal amplification (RT-LAMP) method and a MinION sequencer.We developed the protocol for drying all the reagents for the RT-LAMP in a single reaction tube.Using this system, the CHIKV genome was effectively amplified under isothermal conditions, and used as a...
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