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Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome

29 May 2018

Abstract excerpt

BACKGROUND: A single-tube one-step real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of chikungunya virus (CHIKV) targeting the conserved 6K-E1 target region was developed. The assay was validated with sera collected from a CHIKV outbreak in Senegal in 2015. METHODOLOGY/PRINCIPAL FINDINGS: A novel design approach by combining Principal Component Analysis...

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PMID: 29813065
DOI: 10.1371/journal.pntd.0006448

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