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Simultaneous Detection of Different Zika Virus Lineages via Molecular Computation in a Point-of-Care Assay

14 Dec 2018

Abstract excerpt

We have developed a generalizable “smart molecular diagnostic” capable of accurate point-of-care (POC) detection of variable nucleic acid targets. Our isothermal assay relies on multiplex execution of four loop-mediated isothermal amplification reactions, with primers that are degenerate and redundant, thereby increasing the breadth of targets while reducing the probability of amplification failure. An...

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PMID: 30558136
DOI: 10.3390/v10120714

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