Article
A microfluidic cell-trapping device to study dynamic host-microbe interactions at the single-cell level.
Methods in cell biology - 1 Jan 2018
Toniolo Chiara, Delincé Matthieu, McKinney John D
Abstract excerpt
Single-cell imaging of host-microbe interactions over time is impeded by cellular motility because the cells under scrutiny tend to migrate out of the imaging field. To overcome this technical challenge, we developed a microfluidic platform for imaging hundreds of individual motile phagocytic cells and bacteria within microfluidic traps that restrict their movement. The interaction of trapped host cells and...
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